Long-range upstream and downstream enhancers control distinct subsets of the complex spatiotemporal Sox9 expression pattern. We first used concatenated sequences. Neither of the transcripts were detectable in the absence of cDNA input. Regulation of Germ Cell Migration.
Y TIR gonads examined, either ovaries or ovotestes, as well as B6. Breeding system data followed . The resulting alignments total bp Table S3 shows the numbers of sites per gene. While the pronephros and mesonephros are transient structures that soon degenerate, the metanephros is one of the main sources of the definitive kidney.
AMH gene mutations with a normal AMH serum level are very unusual and should be regarded with suspicion. Finally, the slides were incubated with the avidin conjugated with Cy3 at dilutions and processed for mounting with DAPI as described above.
Fluorescence signals were captured by digital camera as described above. The presence or absence of primordial germ cells, of extragonadal origin, also has a sexually dimorphic relevance.
At 45 days, the ovary is recognizable only because it has not yet undergone testicular differentiation. Factors involved in germ cell meiosis are also important. Germ cell-intrinsic and -extrinsic factors govern meiotic initiation in mouse embryos. In order for the fetal testis to adequately differentiate and secrete masculinizing hormones, not only do all these factors need to be present at sufficient levels in the right cell lineage, but their expression must also be initiated within a narrow time window.